Cloning strategy
Selection of an appropriate method, including Golden Gate, Gibson, restriction-ligation, and modular assembly workflows.
Cloning design for research teams
Build the right construct before you go to the bench.
CloneWise helps researchers plan, review, and troubleshoot molecular cloning projects—so your primers, vectors, and assembly strategy are clear before you order anything.
Based in Germany
Serving researchers worldwide
Confidential project review
Construct design
Checked
- Reading frame verified
- Restriction sites checked
- Assembly logic reviewed
Why CloneWise
Most cloning failures begin at the design stage.
A missing restriction-site check, an incorrect reading frame, or the wrong assembly strategy can cost days of work and another round of ordering.
CloneWise gives you an independent, expert review before your project reaches the bench.
Services
Practical support from idea to bench-ready design.
Plasmid and construct design
Design of vector architecture, promoters, coding sequences, tags, linkers, terminators, and assembly junctions.
Design review and troubleshooting
Independent review of existing plans, primer logic, reading frames, internal sites, compatibility, and likely failure points.
Primer planning
Bench-ready primer sequences with overhangs, assembly logic, expected products, and a clear explanation of each design decision.
Plant molecular biology support
Specialist support for plant expression vectors, CRISPR constructs, fluorescent proteins, and multigene assemblies.
Online consultation
Focused project discussions by Zoom for complex constructs, experimental planning, or troubleshooting.
How it works
From project brief to a clear cloning plan.
Send your brief
Share your target, organism, vector, sequences, and experimental goal.
We design and review
The complete strategy is checked for compatibility, frame, junctions, and practical risks.
Receive your report
You receive annotated files, primer details, and a written explanation of the design.
Go to the bench
Order with confidence and start the experiment with a clear, bench-ready plan.
Packages
Choose the level of cloning design support your project needs.
Each package includes vector-map design, primer design, and a clear cloning protocol up to verification by restriction digestion.
Package 1
Essential Design
€150
For small cloning projects.
- Up to 2 vector maps
- Primer design
- Step-by-step cloning protocol
- Restriction-digest verification plan
Most popular
Package 2
Standard Design
€200
For medium cloning projects.
- Up to 5 vector maps
- Primer design
- Step-by-step cloning protocol
- Restriction-digest verification plan
- 30-minute online consultation
Package 3
Advanced Design
€250
For larger construct design projects.
- Up to 8 vector maps
- Primer design
- Step-by-step cloning protocol
- Restriction-digest verification plan
- 2 × 30-minute online consultations
Package 4
Custom Quote
More than 10 vector maps? Please request a custom quotation.
Request quotationFinal scope and price are confirmed before payment. Complex constructs, urgent timelines, or additional verification strategies may require a custom quotation.
Design before spending
One failed attempt can cost more than a design review.
Primers€25
Reagents€20
Sequencing verification€20
Scientific working time€300
Potential cost€365 + lost time
About CloneWise
Scientific expertise translated into practical cloning decisions.
CloneWise is an independent molecular biology design service founded by a plant scientist with hands-on experience in Golden Gate cloning, multigene assemblies, CRISPR/dCas9 systems, fluorescent protein constructs, and plant expression workflows.
The goal is simple: make complex cloning projects easier to understand, easier to execute, and less likely to fail.
“During my PhD, Bhanu provided invaluable support in designing a Golden Gate cloning strategy for my project. He helped me choose the appropriate vectors and fluorescent proteins, explained the reasoning behind each decision, and guided me through the cloning workflow in great detail. His advice was highly practical and instrumental in the successful completion of my cloning experiments.”
— Gihwan Kim
Start a project
Tell us what you want to build.
For package requests, please use the project details form. You can also email a short project description if you prefer.
Project details form
Open project form
Paste sequences, describe templates, or upload sequence files.
Email
hello@clonewise.de
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