CloneWise

CRISPR & genome editing design

Plant CRISPR construct design

Plant-focused CRISPR-Cas9 design support, from target choice and sgRNA selection to cloning logic, promoter/scaffold planning, and genotyping confirmation strategy.

DESIGN WORKFLOW Target gene sgRNA design Off-target check Cloning plan Genotyping CONSTRUCT DESIGN OUTPUT sgRNA CASSETTE Cas9 CASSETTE U6/U3 promoter spacer scaffold promoter Cas9 terminator Example design output: sgRNA cassette and Cas9 expression cassette

What this service is for

This service is for labs planning plant CRISPR knockout or single-gene editing experiments and needing a clear design before starting bench work.

  • Target-gene or target-locus review
  • sgRNA candidate screening, comparison, and selection with clear reasoning
  • Off-target screening and interpretation against the reference genome
  • Cut-site and reading-frame summary for each target, where applicable
  • CRISPR cloning plan with promoter, scaffold, and vector-design logic
  • Genotyping primers and a confirmation strategy

What you receive

  • A design summary for up to 2 target genes or loci
  • Recommended sgRNA candidates with the reasoning for each
  • An annotated cloning plan for the selected construct strategy
  • Genotyping primer sequences and the expected confirmation logic
  • One 30-minute online consultation to discuss the design

Scope note

This service covers CRISPR construct design and planning. Experimental cloning, plant transformation, genome editing experiments, biological validation, and confirmed mutant generation are not included.

Ready to plan your CRISPR design?

Send a short inquiry with your target gene or project goal. Full sequences are not needed at the first step.

Start a CRISPR design inquiry
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