CloneWise

IN SILICO DESIGN SUPPORT

Cloning & construct design

In silico plasmid and construct design for clear cloning plans, primer details, assembly logic, and verification workflows before bench work.

DESIGN WORKFLOW Construct goal Vector design Primer design Assembly plan Verification SUPPORTED METHODS Golden Gate Gibson Gateway Restriction

What this service is for

This service is for researchers who need a clear plasmid-level design before ordering primers, gene fragments, or starting cloning.

  • Plant overexpression constructs
  • Bacterial expression constructs for protein purification
  • Fluorescent protein or tag-fusion constructs
  • Golden Gate, Gibson, Gateway, or restriction cloning designs
  • Related construct sets or multigene assembly planning

What you receive

  • Vector map or construct-design summary
  • Primer sequences and cloning-junction logic
  • Step-by-step assembly workflow
  • Verification plan, including a sequencing or diagnostic-check strategy
  • Online consultation included, depending on the selected package

Package scope

  • Basic Construct Design: up to 5 related simple plasmid designs within one project
  • Extended Construct Design: up to 10 related plasmid designs within one project, depending on complexity
  • Very large, unrelated, highly repetitive, or technically complex designs may be quoted separately

Ready to plan your cloning project?

Send a short inquiry with your construct goal, vector system, or cloning question. Full sequences are not needed at the first step.

Start a cloning project
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